Auckland Genomics

We offer a wide range of NextGen DNA/RNA sequencing (NGS), Sanger sequencing and gene expression services.

Equipment

Applied Biosystems 3130XL Genetic Analyzer

  • 16 lane Sanger sequencing and genotyping

Chromium 10x

  • scRNAseq and linked read genome libraries

ThermoFisher Clariom Arrays

  • Clariom S and D cartridge array for gene expression

Real-Time qPCR and Digital Droplet PCR

  • ThermoFisher QuantStudio 5 Real-Time PCR System
  • Bio-Rad QX200Droplet Digital ddPCR System

DNA/RNA QC instruments

  • BioAnalyzer, Qubit, plate readers, Fragment Analyzer, BluePippin, NanoDrop, Covaris

Illumina sequencing

  • A full range of Illumina sequencing options are available either on-site or through our partners

Nanopore sequencing

  • Long read sequencing using minION and Flongle flow cells (R9.4 and R10)

Services

  • Illumina sequencing
  • Nanopore sequencing (long reads or amplicons)
  • Chromium 10x scRNAseq and linked read genome libraries
  • NGS library construction
  • Nucleic acid extraction, QC and quantification
  • Clariom gene expression arrays
  • Sanger sequencing and genotyping.

How we can help you

NGS project design, method development, cost information and/or sample requirements

Contact us at genomics@auckland.ac.nz to discuss your project design. Please include the following information in your email:

  • your name, institution, and contact phone number
  • brief hypothesis you desire to test or your goal in doing the project
  • the type of sequencing you want (Illumina/Nanopore),
  • the number of reads OR amount of sequencing data per sample you require
  • what type of library prep is required (i.e. rRNA depletion, Nextera library, etc)
  • for illumina projects what read length you need (i.e. 1 x75bp, 2x 250bp, etc)

Cost and sample requirement information will be supplied once your project has been designed.

When you have a project numbers and are ready to submit samples, use our packing guidelines:

Sanger sequencing and genotyping

All cost and sample requirement information for Sanger sequencing and genotyping is available through iLab.

Training requirements

Training and booking for all instruments is available through iLab.

Our people

Auckland genomics instument